94. Preparation of Glucose-sensitive and Fluorescent Micelles via Combination of Photoinitiated Polymerization and Chemenzymatic Tranesterification for Controlled Release of Insulin
writer：Xiangxiang Du, Guohua Jiang*, Lei Li, Wentong Yang, Hua Chen, Yongkun Liu, Qin Huang
keywords：glucose-sensitive; fluorescence; photopolymerization; chemenzymatic tranesterification; drug release
source：期刊
specific source：Journal of Applied Polymer Science, 2016, 133(9), 43026
Issue time：2016年
Glucose-sensitive and fluorescence copolymer micelles were designed and prepared via combination of photoinitiated polymerization and enzymatic transesterification. The water-soluble photoinitiator and emulsifier 2-oxooctanoic acid (2-OOA) self-polymerized dimer molecules under UV irradiation characterized by mass spectrum (MS). The fluorescence dye, 9-anthracene alcohol (9-AMOH) and biocompatible hydrophilic chains, polyethylene glycol (PEG-OH), were introduced to the polymer chains during the photopolymerization and enzymatic transesterification process. The as-prepared copolymers were confirmed by 1H NMR、FT-IR、TEM and DLS. The resultant copolymers exhibited the excellent glucose sensitivity and stability against protein. The optical fluorescence properties of copolymer micelles were investigated using fluorescence spectrophotometer and fluorescence microscope and confocal laser scanning microscopy (CLSM). Due to the amphiphiilic feature, the micelles could be self-assembled and used to load insulin. The controlled release of insulin were evaluated triggered by glucose in vitro. This work provided a new strategy for fabricating functional carriers as self-regulated insulin release systems.
