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The chiral effects on the responses of osteoblastic cells to the polymeric substrates
作者:Qiangying Yi, Xiantao Wen, Li Li, Bin He*, Yu Nie, Yao Wu, Zhirong Zhang, Zhongwei Gu*
關(guān)鍵字:Chirality, Stereocomplex, Polylactide
論文來(lái)源:期刊
具體來(lái)源:Eur. Polym. J., 2009, 45:1970-1978
發(fā)表時(shí)間:2009年

In order to study the chiral effects of polylactides on responses of osteoblastic cells, poly(Llactide) (PLLA), poly(D-lactide) (PDLA), poly(DL-lactide) (PDLLA) and the stereocomplex of PLLA and PDLLA (SC) films with different stereoforms were prepared. The surface properties of the four polylactide films were tested and the osteoblastic ROS 17/2.8 cells were cultured on the films. The protein adsorption behaviors of fibrinogen and bovine serum albumin on films were studied. The cell proliferation, total protein amount, DNA content and alkaline phosphatase activity of osteoblastic ROS 17/2.8 cells were evaluated. The results showed that the protein adsorption was dependant on the type of proteins. The observation of cell morphologies revealed that the PDLA film provide an unfavorable surface for cell attachment. The total protein amount, DNA content and ALP activity were closely related to the stereoforms of polylactide films. All the levels of total protein amount, DNA content and ALP activity of ROS 17/2.8 cells on PDLA film were decreased. The racemic stereocomplex of PLLA and PDLA showed relatively higher positive effects on both cell growth and proliferation.

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