Micellar electrokinetic chromatography analysis of tetrahydrogestrinone and related anabolic androgenic steroids.
作者：Chen, Hong-Xu Huang, Mu-Hua Zhang, Xin-Xiang.
關鍵字：tetrahydrogestrinone androgen steroid micellar electrokinetic chromatog
論文來源：期刊
具體來源：Analytical Methods
發(fā)表時間：2013年
Tetrahydrogestrinone and related anabolic androgenic steroids (AASs) have been used extensively for performance enhancement (doping) in sports due to their ability to accelerate protein synthesis and improve phys. performance (F. C. W. Wu, Clin. Chem., 1997, 43, 1289-1292). The sepn. of these AASs was performed by micellar electrokinetic chromatog. (MEKC) using borate/borax buffer (200/50 mM, pH 8.6), with sodium cholate (40 mM) as a chiral additive. The analytes included two endogenous steroid hormones (testosterone and epitestosterone) and five synthetic anabolic steroids (methyltestosterone, nandrolone, gestrinone, dihydrogestrinone and tetrahydrogestrinone). Capillary electrophoresis parameters such as the concn. of additives, injection time, temp., and applied voltage were investigated to improve the sepn. efficiency. A complete sepn. was achieved in less than 16 min under the optimized conditions. The RSDs of peak area and migration time were below 3.9% and 0.34%, resp. The limit of detection (LOD) between 240 and 570 ng mL-1 was obtained for each of the pure stds. with a photodiode array (PDA) detector. Lower LODs could be reached when combining with preconcn. After liq.-liq. extn., the recoveries of spiked urine samples were in the range from 88% to 99%. [on SciFinder(R)]