【[Scientific Reports]】Hollow Au-Ag Nanoparticles Labeled Immunochromatography Strip for Highly Sensitive Detection of Clenbuterol
作者：Wang, J.; Zhang, L.; Huang, Y.*; Dandapat, A.; Dai, L.; Zhang, G.; Lu, X.; Zhang, J.; Lai, W*.; Chen,T*.
關鍵字：lateral-flow immunochromatographic test strip
論文來源：期刊
具體來源：Scientific Reports,2017,2045-2322
發表時間：2017年
The probe materials play a significant
role in improving the detection efficiency and sensitivity of lateral-flow immunochromatographic
test strip (ICTS). Unlike conventional ICTS assay usually uses single-component,
solid noble metal particles (e.g., gold nanoparticles) as labeled probes, in
our present study, a bimetallic, hollow Au-Ag nanoparticles (NPs) labeled ICTS
was successfully developed for the detection of clenbuterol (CLE).
The hollow Au-Ag NPs with different Au/Ag mole ratio and tunable size were
synthesized by varying the volume ratio of [HAuCl4]: [Ag NPs] via the galvanic
replacement reaction. The surface of hollow Ag-Au NPs was functionalized with
11-mercaptoundecanoic acid (MUA) for further covalently bonded with anti-CLE
monoclonal antibody to acquire highly stable hollow Au-Ag NPs probe.
Overall size of the Au-Ag NPs, size of the holes within individual NPs and also
Au/Ag mole ratio have been systematically optimized to amplify both the visual inspection
signals and the quantitative data (optical densities) recorded using a bioassay
reader. The sensitivity of optimized hollow Au-Ag NPs
probes for the detection of CLE has been
achieved even as low as 2 ppb in a short time (within 15 min), which is
superior over the detection performance of conventional test strip using Au NPs.
The optimized hollow
Au-Ag NPs labeled test strip can be used as an ideal candidate
for the rapid screening of CLE in food samples.