Gold Nanorods with Silica Shell and PAMAM Dendrimers for Efficient Photothermal therapy and Low Toxic Co-Delivery of Anti-Cancer Drug and siRNA
作者：Qianyu Zhang, Li Wang, Yajun Jiang, Wei Gao, Yinsong Wang, Xiaoying Yang*, Xinlin Yang, Zunfeng Liu*
關(guān)鍵字：gold nanorods, photothermal therapy, drug delivery, gene delivery
論文來源：期刊
具體來源：Adv. Mater. Interfaces 2017, 1701166 (1-9). DOI: 10.1002/admi.201701166.
發(fā)表時(shí)間：2017年
The multifunctional nanocomposites with grafting polyamidoamine (PAMAM) dendrimers
up to third generation (G3) was grown onto the surface of mesoporous silica-coated gold nanorods (AuNRs@SiO2) via a divergent technique. The resultant AuNRs@SiO2-G3
nanocomposites with uniform size and excellent stability not only enable their utilization as targeted contrast agents for photothermal cancer therapy, but also serve as scaffolds for the intracellular delivery of anti-cancer drug and small interfering RNA (siRNA) to enhance the efficiency of cancer therapy. The controlled doxorubicin (DOX) release from AuNRs@SiO2-G3 nanocomposites is significantly improved under lower pH condition and near-infrared (NIR) laser irradiation. The Bcl-2 targeted siRNA is transfected into tumor
cells and induce knockdown of the protein expression, which is confirmed by western blot assays. Furthermore, the complementary effect of chemo-and gene therapy to MCF-7 cells for improved photothermal therapy is demonstrated by MTT assay. The DOX and siRNA co-loaded AuNRs@SiO2-G3 nanocomposites showed much lower cytotoxicity compared to the DOX, relsuting in low toxicity to nomal tissues. The multifunctional nanocomposites have potential application as nanoplatforms to integrate the photothermal-chemo-gene tumor therapy with high efficiency.