報告主題：DNA與聚陽離子復合物: 從基因傳遞載體到原始細胞模型
報告人：梁德海 教授
主持人：陳永明 教授
時間：2015年12月29日（星期二）上午09:30-10:30
地點：豐盛堂A403
報告摘要：
研究DNA/RNA 與聚陽離子的靜態(tài)和動態(tài)復合行為對于探討聚電解質(zhì)的相互作用機理，開發(fā)高效基因傳遞載體，以及研究原始細胞的動態(tài)模型都有重要意義。在靜態(tài)復合中，我們重點探討了DNA復合物的長程動力學行為，發(fā)現(xiàn)歧化是影響復合物動力學的主要因素。通過調(diào)控歧化速率，構(gòu)筑了動力學控制的膠囊結(jié)構(gòu)。在動態(tài)復合中，首先模擬血液流動引起的剪切作用，探討了外加剪切力對DNA復合物穩(wěn)定性的影響，并據(jù)此開發(fā)了具有肺靶向功能的siRNA傳遞載體。 接下來，利用電場來調(diào)控DNA與聚陽離子在非平衡態(tài)的復合行為。結(jié)果表明，二者形成的復合物發(fā)生了周期性的內(nèi)分區(qū)化，尺寸和形狀的動態(tài)漲落，混沌式的增長和融合等許多與生命活動類似的行為。在周期性的內(nèi)分區(qū)化過程中還伴隨著物質(zhì)的運輸，主要表現(xiàn)為聚陽離子含量的減少。我們用電場力學引起的聚電解質(zhì)復合物的解散，繼而引起的滲透壓變化來解釋內(nèi)分區(qū)化行為。這些結(jié)果都表明在非平衡態(tài)下研究復合物粒子的動態(tài)行為對于開發(fā)原始細胞，探索生命起源具有指導意義。
報告人簡介：
EDucation
2001 Ph.D. Department of Chemistry, State University of New York at Stony Brook, NY, USA (Thesis Advisor: Benjamin Chu)
1996 M.S. (Candidate), Department of Chemistry, Nankai Unversity, Tianjin, China
1994 B.S. Department of Environmental Sciences, Nankai Unversity, Tianjin, China
Employment
2012-present: Professor, College of Chemistry, Peking University, Beijing, China
2006-2012: Associate Professor, College of Chemistry, Peking University, Beijing, China
2001-2005: Postdoctoral Research Associate, Department of Chemistry, State University of New York at Stony Brook, NY, USA
Research Interests
1. Electric field excitation and non-equilibrium dynamics in Peptide/DNA protocells
2. Anisotropic assembly regulated by macromolecular crowding and confinement
3. Peptide mediated endocytosis of biomembrane
4. Enzyme reaction in polyelectrolyte complex
5. Lung-targeting siRNA delivery vehicles
Teaching Reponsibility
Polymer physics: structures and properties, 2007-present
Today’s Chemistry, 2010-present
Comprehensive Experiments: DNA complex, 2009-2012
Literature Seminar, 2007-2015
Honors &Awards
2015 The 9th Feng Xinde Polymer Prize, Elsevier
2010 Winner of Education Ministry''''''''s New Century Excellent Talents Supporting Plan
2001 President’s Award to Distinguished Doctoral Students, SUNY at Stony Brook
2001 Lee Myer’s Outstanding Graduate Student Award, SUNY at Stony Brook
2000 Excellence in Research Award, Sigma Xi, the Scientific Research Society
Publicatons
110 Papers, 2 US patents and 4 Chinese patents.